Although we have not tested PneumaCult™ media to be used with murine epithelial cells, there are publications that report this application.
Combine gently right before transferring cells to a conical tube that contains 10 mL of pre-warmed clean medium. Notice: Wash the pipette idea and cryovial Together with the wash medium not less than 3 periods that can help enhance Restoration.
If utilizing Gentle Cell Dissociation Reagent (GCDR), the length of GCDR treatment will have to be optimized. Incubate organoids with GCDR for eight -10 minutes at 37°C and utilize a P1000 pipette to gently pipette up and down, and visually inspect if organoids crack up.
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Cross-area histology accompanied by hematoxylin and eosin (H&E) staining to evaluate the thickness of the tiny or substantial airway epithelium
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Take note: Continue to antibody staining if doing Are living cell Assessment immediately after this step. Alternatively, proceed to fixable dye staining and PFA fixation.
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Carry out 줄기세포 지방이식 cell counts making use of an automatic cell counter. Observe: Small viability can be optimized from the stem cell clinic length of collagenase or ACCUTASE™ incubation. It may also be optimized by reducing the volume of 줄기세포 지방이식 triturations.
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